Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment.
Identifieur interne : 001989 ( Main/Exploration ); précédent : 001988; suivant : 001990Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment.
Auteurs : Julie M D. Paye [États-Unis] ; Anna Guseva [États-Unis] ; Sarah K. Hammer [États-Unis] ; Erica Gjersing [États-Unis] ; Mark F. Davis [États-Unis] ; Brian H. Davison [États-Unis] ; Jessica Olstad [États-Unis] ; Bryon S. Donohoe [États-Unis] ; Thanh Yen Nguyen [États-Unis] ; Charles E. Wyman [États-Unis] ; Sivakumar Pattathil [États-Unis] ; Michael G. Hahn [États-Unis] ; Lee R. Lynd [États-Unis]Source :
- Biotechnology for biofuels [ 1754-6834 ] ; 2016.
Abstract
BACKGROUND
Feedstock recalcitrance is the most important barrier impeding cost-effective production of cellulosic biofuels. Pioneer commercial cellulosic ethanol facilities employ thermochemical pretreatment and addition of fungal cellulase, reflecting the main research emphasis in the field. However, it has been suggested that it may be possible to process cellulosic biomass without thermochemical pretreatment using thermophilic, cellulolytic bacteria. To further explore this idea, we examine the ability of various biocatalysts to solubilize autoclaved but otherwise unpretreated cellulosic biomass under controlled but not industrial conditions.
RESULTS
Carbohydrate solubilization of mid-season harvested switchgrass after 5 days ranged from 24 % for Caldicellulosiruptor bescii to 65 % for Clostridium thermocellum, with intermediate values for a thermophilic horse manure enrichment, Clostridium clariflavum, Clostridium cellulolyticum, and simultaneous saccharification and fermentation (SSF) featuring a fungal cellulase cocktail and yeast. Under a variety of conditions, solubilization yields were about twice as high for C. thermocellum compared to fungal cellulase. Solubilization of mid-season harvested switchgrass was about twice that of senescent switchgrass. Lower yields and greater dependence on particle size were observed for Populus as compared to switchgrass. Trends observed from data drawn from six conversion systems and three substrates, including both time course and end-point data, were (1) equal fractional solubilization of glucan and xylan, (2) no biological solubilization of the non-carbohydrate fraction of biomass, and (3) higher solubilization for three of the four bacterial cultures tested as compared to the fungal cellulase system. Brief (5 min) ball milling of solids remaining after fermentation of senescent switchgrass by C. thermocellum nearly doubled carbohydrate solubilization upon reinnoculation as compared to a control without milling. Greater particle size reduction and solubilization were observed for milling of partially fermented solids than for unfermented solids. Physical disruption of cellulosic feedstocks after initiation of fermentation, termed cotreatment, warrants further study.
CONCLUSIONS
While the ability to achieve significant solubilization of minimally pretreated switchgrass is widespread, a fivefold difference between the most and least effective biocatalyst-feedstock combinations was observed. Starting with nature's best biomass-solubilizing systems may enable a reduction in the amount of non-biological processing required, and in particular substitution of cotreatment for pretreatment.
DOI: 10.1186/s13068-015-0412-y
PubMed: 26759604
PubMed Central: PMC4709877
Affiliations:
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Paye</name><affiliation wicri:level="2"><nlm:affiliation>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Guseva, Anna" sort="Guseva, Anna" uniqKey="Guseva A" first="Anna" last="Guseva">Anna Guseva</name><affiliation wicri:level="2"><nlm:affiliation>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Hammer, Sarah K" sort="Hammer, Sarah K" uniqKey="Hammer S" first="Sarah K" last="Hammer">Sarah K. 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Lynd</name><affiliation wicri:level="2"><nlm:affiliation>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26759604</idno><idno type="pmid">26759604</idno><idno type="doi">10.1186/s13068-015-0412-y</idno><idno type="pmc">PMC4709877</idno><idno type="wicri:Area/Main/Corpus">001965</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001965</idno><idno type="wicri:Area/Main/Curation">001965</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001965</idno><idno type="wicri:Area/Main/Exploration">001965</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment.</title><author><name sortKey="Paye, Julie M D" sort="Paye, Julie M D" uniqKey="Paye J" first="Julie M D" last="Paye">Julie M D. Paye</name><affiliation wicri:level="2"><nlm:affiliation>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Guseva, Anna" sort="Guseva, Anna" uniqKey="Guseva A" first="Anna" last="Guseva">Anna Guseva</name><affiliation wicri:level="2"><nlm:affiliation>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Hammer, Sarah K" sort="Hammer, Sarah K" uniqKey="Hammer S" first="Sarah K" last="Hammer">Sarah K. Hammer</name><affiliation wicri:level="2"><nlm:affiliation>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Gjersing, Erica" sort="Gjersing, Erica" uniqKey="Gjersing E" first="Erica" last="Gjersing">Erica Gjersing</name><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Colorado</region></placeName><wicri:cityArea>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden</wicri:cityArea></affiliation></author><author><name sortKey="Davis, Mark F" sort="Davis, Mark F" uniqKey="Davis M" first="Mark F" last="Davis">Mark F. Davis</name><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Colorado</region></placeName><wicri:cityArea>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden</wicri:cityArea></affiliation></author><author><name sortKey="Davison, Brian H" sort="Davison, Brian H" uniqKey="Davison B" first="Brian H" last="Davison">Brian H. Davison</name><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Olstad, Jessica" sort="Olstad, Jessica" uniqKey="Olstad J" first="Jessica" last="Olstad">Jessica Olstad</name><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Colorado</region></placeName><wicri:cityArea>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden</wicri:cityArea></affiliation></author><author><name sortKey="Donohoe, Bryon S" sort="Donohoe, Bryon S" uniqKey="Donohoe B" first="Bryon S" last="Donohoe">Bryon S. Donohoe</name><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Colorado</region></placeName><wicri:cityArea>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden</wicri:cityArea></affiliation></author><author><name sortKey="Nguyen, Thanh Yen" sort="Nguyen, Thanh Yen" uniqKey="Nguyen T" first="Thanh Yen" last="Nguyen">Thanh Yen Nguyen</name><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, Riverside, 1084 Columbia Ave, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, Riverside, 1084 Columbia Ave, Riverside</wicri:cityArea></affiliation></author><author><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, Riverside, 1084 Columbia Ave, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, Riverside, 1084 Columbia Ave, Riverside</wicri:cityArea></affiliation></author><author><name sortKey="Pattathil, Sivakumar" sort="Pattathil, Sivakumar" uniqKey="Pattathil S" first="Sivakumar" last="Pattathil">Sivakumar Pattathil</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens GA, 30602 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens GA</wicri:regionArea><wicri:noRegion>Athens GA</wicri:noRegion></affiliation></author><author><name sortKey="Hahn, Michael G" sort="Hahn, Michael G" uniqKey="Hahn M" first="Michael G" last="Hahn">Michael G. Hahn</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens GA, 30602 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens GA</wicri:regionArea><wicri:noRegion>Athens GA</wicri:noRegion></affiliation></author><author><name sortKey="Lynd, Lee R" sort="Lynd, Lee R" uniqKey="Lynd L" first="Lee R" last="Lynd">Lee R. Lynd</name><affiliation wicri:level="2"><nlm:affiliation>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge</wicri:cityArea></affiliation></author></analytic><series><title level="j">Biotechnology for biofuels</title><idno type="ISSN">1754-6834</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Feedstock recalcitrance is the most important barrier impeding cost-effective production of cellulosic biofuels. Pioneer commercial cellulosic ethanol facilities employ thermochemical pretreatment and addition of fungal cellulase, reflecting the main research emphasis in the field. However, it has been suggested that it may be possible to process cellulosic biomass without thermochemical pretreatment using thermophilic, cellulolytic bacteria. To further explore this idea, we examine the ability of various biocatalysts to solubilize autoclaved but otherwise unpretreated cellulosic biomass under controlled but not industrial conditions.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Carbohydrate solubilization of mid-season harvested switchgrass after 5 days ranged from 24 % for Caldicellulosiruptor bescii to 65 % for Clostridium thermocellum, with intermediate values for a thermophilic horse manure enrichment, Clostridium clariflavum, Clostridium cellulolyticum, and simultaneous saccharification and fermentation (SSF) featuring a fungal cellulase cocktail and yeast. Under a variety of conditions, solubilization yields were about twice as high for C. thermocellum compared to fungal cellulase. Solubilization of mid-season harvested switchgrass was about twice that of senescent switchgrass. Lower yields and greater dependence on particle size were observed for Populus as compared to switchgrass. Trends observed from data drawn from six conversion systems and three substrates, including both time course and end-point data, were (1) equal fractional solubilization of glucan and xylan, (2) no biological solubilization of the non-carbohydrate fraction of biomass, and (3) higher solubilization for three of the four bacterial cultures tested as compared to the fungal cellulase system. Brief (5 min) ball milling of solids remaining after fermentation of senescent switchgrass by C. thermocellum nearly doubled carbohydrate solubilization upon reinnoculation as compared to a control without milling. Greater particle size reduction and solubilization were observed for milling of partially fermented solids than for unfermented solids. Physical disruption of cellulosic feedstocks after initiation of fermentation, termed cotreatment, warrants further study.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>While the ability to achieve significant solubilization of minimally pretreated switchgrass is widespread, a fivefold difference between the most and least effective biocatalyst-feedstock combinations was observed. Starting with nature's best biomass-solubilizing systems may enable a reduction in the amount of non-biological processing required, and in particular substitution of cotreatment for pretreatment.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">26759604</PMID><DateCompleted><Year>2016</Year><Month>01</Month><Day>13</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1754-6834</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Biotechnology for biofuels</Title><ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation></Journal><ArticleTitle>Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment.</ArticleTitle><Pagination><MedlinePgn>8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13068-015-0412-y</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Feedstock recalcitrance is the most important barrier impeding cost-effective production of cellulosic biofuels. Pioneer commercial cellulosic ethanol facilities employ thermochemical pretreatment and addition of fungal cellulase, reflecting the main research emphasis in the field. However, it has been suggested that it may be possible to process cellulosic biomass without thermochemical pretreatment using thermophilic, cellulolytic bacteria. To further explore this idea, we examine the ability of various biocatalysts to solubilize autoclaved but otherwise unpretreated cellulosic biomass under controlled but not industrial conditions.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Carbohydrate solubilization of mid-season harvested switchgrass after 5 days ranged from 24 % for Caldicellulosiruptor bescii to 65 % for Clostridium thermocellum, with intermediate values for a thermophilic horse manure enrichment, Clostridium clariflavum, Clostridium cellulolyticum, and simultaneous saccharification and fermentation (SSF) featuring a fungal cellulase cocktail and yeast. Under a variety of conditions, solubilization yields were about twice as high for C. thermocellum compared to fungal cellulase. Solubilization of mid-season harvested switchgrass was about twice that of senescent switchgrass. Lower yields and greater dependence on particle size were observed for Populus as compared to switchgrass. Trends observed from data drawn from six conversion systems and three substrates, including both time course and end-point data, were (1) equal fractional solubilization of glucan and xylan, (2) no biological solubilization of the non-carbohydrate fraction of biomass, and (3) higher solubilization for three of the four bacterial cultures tested as compared to the fungal cellulase system. Brief (5 min) ball milling of solids remaining after fermentation of senescent switchgrass by C. thermocellum nearly doubled carbohydrate solubilization upon reinnoculation as compared to a control without milling. Greater particle size reduction and solubilization were observed for milling of partially fermented solids than for unfermented solids. Physical disruption of cellulosic feedstocks after initiation of fermentation, termed cotreatment, warrants further study.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">While the ability to achieve significant solubilization of minimally pretreated switchgrass is widespread, a fivefold difference between the most and least effective biocatalyst-feedstock combinations was observed. Starting with nature's best biomass-solubilizing systems may enable a reduction in the amount of non-biological processing required, and in particular substitution of cotreatment for pretreatment.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Paye</LastName><ForeName>Julie M D</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guseva</LastName><ForeName>Anna</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hammer</LastName><ForeName>Sarah K</ForeName><Initials>SK</Initials><AffiliationInfo><Affiliation>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gjersing</LastName><ForeName>Erica</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Davis</LastName><ForeName>Mark F</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Davison</LastName><ForeName>Brian H</ForeName><Initials>BH</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Olstad</LastName><ForeName>Jessica</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Donohoe</LastName><ForeName>Bryon S</ForeName><Initials>BS</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nguyen</LastName><ForeName>Thanh Yen</ForeName><Initials>TY</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, Riverside, 1084 Columbia Ave, Riverside, CA USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wyman</LastName><ForeName>Charles E</ForeName><Initials>CE</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, Riverside, 1084 Columbia Ave, Riverside, CA USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pattathil</LastName><ForeName>Sivakumar</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens GA, 30602 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hahn</LastName><ForeName>Michael G</ForeName><Initials>MG</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA ; Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens GA, 30602 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lynd</LastName><ForeName>Lee R</ForeName><Initials>LR</Initials><AffiliationInfo><Affiliation>Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 USA ; BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge, Oak Ridge, TN USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>01</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biotechnol Biofuels</MedlineTA><NlmUniqueID>101316935</NlmUniqueID><ISSNLinking>1754-6834</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biological solubilization</Keyword><Keyword MajorTopicYN="N">Cotreatment</Keyword><Keyword MajorTopicYN="N">Lignocellulose</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>08</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>12</Month><Day>04</Day></PubMedPubDate><PubMedPubDate 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Lynd</name><name sortKey="Nguyen, Thanh Yen" sort="Nguyen, Thanh Yen" uniqKey="Nguyen T" first="Thanh Yen" last="Nguyen">Thanh Yen Nguyen</name><name sortKey="Olstad, Jessica" sort="Olstad, Jessica" uniqKey="Olstad J" first="Jessica" last="Olstad">Jessica Olstad</name><name sortKey="Pattathil, Sivakumar" sort="Pattathil, Sivakumar" uniqKey="Pattathil S" first="Sivakumar" last="Pattathil">Sivakumar Pattathil</name><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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